Efficient monitoring of protein ubiquitylation levels using TUBEs-based microarrays.
FEBS Lett
; 590(16): 2748-56, 2016 08.
Article
en En
| MEDLINE
| ID: mdl-27410252
ABSTRACT
Analyzing protein ubiquitylation changes during physiological or pathological processes is challenging due to its high reversibility and dynamic turnover of modified targets. We have developed a protein microarray to assess endogenous ubiquitylation levels from cell cultures, employing tandem ubiquitin-binding entities (TUBEs) with three or four ubiquitin-associated (UBA) domains as capture probes. Adriamycin (ADR)-stimulated MCF7 cells were used to differentiate protein ubiquitylation levels between cells that are sensitive or resistant to ADR treatment. We show that TUBEs-based microarrays can be used for the analysis of cellular processes regulated by ubiquitylation and for the detection of pathologies with aberrant ubiquitylation levels.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas
/
Análisis por Matrices de Proteínas
/
Ubiquitinación
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2016
Tipo del documento:
Article
País de afiliación:
España