Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity.
Acta Paediatr
; 106(3): 430-437, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-27886403
ABSTRACT
AIM:
Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation.METHODS:
Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 µL of aspirate.RESULTS:
An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results.CONCLUSION:
Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Síndrome de Dificultad Respiratoria del Recién Nacido
/
Esfingomielinas
/
Pulmón
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
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Prognostic_studies
/
Risk_factors_studies
Límite:
Female
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Humans
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Male
/
Newborn
Idioma:
En
Revista:
Acta Paediatr
Año:
2017
Tipo del documento:
Article
País de afiliación:
Dinamarca