Reverse Phase Compatible TLC-Bioautography for Detection of Tyrosinase Inhibitors.
Phytochem Anal
; 28(2): 101-105, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-27910199
ABSTRACT
INTRODUCTION:
Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved.OBJECTIVES:
To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds.METHODS:
Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The gel turns into a brown "skin like" colour due to tyrosinase catalysed oxidation of l-tyrosine. The inhibitors are visualised as clear spots against a brown coloured background.RESULTS:
The assay was able to localise cinnamaldehyde in Cinnamomum cassia essential oil, as its main constituent with known tyrosinase inhibition properties. The assay allowed the detection of 0.03% (w/w) of kojic acid co-spotted with a methanolic extract of Sphaeralcea bonariensis and chromatographed on RP-TLC.CONCLUSION:
The developed assay is able to detect, with high sensitivity, tyrosinase inhibitors present in complex matrices that were chromatographed in RP-TLC. Results can be easily read by colour change, inhibitors appear as clear spots in a darker background. Copyright © 2016 John Wiley & Sons, Ltd.Palabras clave
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Cromatografía en Capa Delgada
/
Monofenol Monooxigenasa
/
Inhibidores Enzimáticos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Phytochem Anal
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Argentina