MiR-23b controls TGF-ß1 induced airway smooth muscle cell proliferation via direct targeting of Smad3.

Chen, Ming; Shi, Jianting; Zhang, Wei; Huang, Linjie; Lin, Xiaoling; Lv, Zhiqiang; Zhang, Wei; Liang, Ruiyun; Jiang, Shanping

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MiR-23b controls TGF-ß1 induced airway smooth muscle cell proliferation via direct targeting of Smad3.

Chen, Ming; Shi, Jianting; Zhang, Wei; Huang, Linjie; Lin, Xiaoling; Lv, Zhiqiang; Zhang, Wei; Liang, Ruiyun; Jiang, Shanping.

Pulm Pharmacol Ther; 42: 33-42, 2017 02.

Artículo en Inglés | MEDLINE | ID: mdl-28062322

BACKGROUND:

MicroRNAs are small yet versatile gene tuners that regulate a variety of cellular processes, including cell growth and proliferation. Here we report that miR-23b inhibited airway smooth muscle cells (ASMCs) proliferation through directly targeting of Smad3.

METHODS:

We obtained ASMCs by laser capture microdissection of normal and asthmatic mice lung tissues. Mice ASMCs were cultured and induced by TGF-ß1. The implication between TGF-ß1 and miR-23b in ASMCs were detected by RT-PCR. The effects of miR-23b on ASMCs proliferation and apoptosis were assessed by transient transfection of miR-23b mimics and inhibitor. The expression of Smad3 in ASMCs were detected by RT-PCR and Western blotting analysis. Dual-Luciferase Reporter Assay System will be applied to identify whether Smad3 is a target gene of miR-23b.

RESULTS:

TGF-ß1 and miR-23b mRNA expression of in-situ bronchial ASMCs collected by laser capture microdissection were increased in asthmatic mice compared to non-asthma controls. This is accompanied by an increase in miR-23b mRNA expression in TGF-ß1 induced ASMCs. miR-23b up-regulation significantly inhibited TGF-ß1-induced ASMCs proliferation and promoted apoptosis. MiR-23b negatively regulates the expression of Smad3 in ASMCs. Dual-Luciferase Reporter Assay System demonstrated that Smad3 was a direct target of miR-23b.

CONCLUSIONS:

MiR-23b may function as an inhibitor of asthma airway remodeling by suppressing ASMCs proliferation via direct targeting of Smad3.

Asunto(s)

Proliferación Celular/genética MicroARNs/genética Proteína smad3/genética Factor de Crecimiento Transformador beta1/metabolismo Remodelación de las Vías Aéreas (Respiratorias)/genética Animales Apoptosis/genética Asma/genética Asma/fisiopatología Western Blotting Modelos Animales de Enfermedad Femenino Ratones Ratones Endogámicos BALB C Miocitos del Músculo Liso/metabolismo Reacción en Cadena de la Polimerasa de Transcriptasa Inversa Regulación hacia Arriba

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MiR-23b controls TGF-ß1 induced airway smooth muscle cell proliferation via direct targeting of Smad3.

BACKGROUND:

METHODS:

RESULTS:

CONCLUSIONS:

Asunto(s)