Local myogenic pulp-derived cell injection enhances craniofacial muscle regeneration in vivo.
Orthod Craniofac Res
; 20(1): 35-43, 2017 Feb.
Article
en En
| MEDLINE
| ID: mdl-28102011
ABSTRACT
OBJECTIVES:
To enhance myogenic differentiation in pulp cells isolated from extracted premolars by epigenetic modification using a DNA demethylation agent, 5-aza-2'-deoxycytidine (5-Aza), and to evaluate the potent stimulatory effect of 5-Aza-treated pulp cell injection for craniofacial muscle regeneration in vivo. SETTING AND SAMPLE POPULATION Pulp cells were isolated from premolars extracted for orthodontic purposes from four adults (age range, 18-22.1 years). MATERIAL ANDMETHODS:
Levels of myogenic differentiation and functional contraction response in vitro were compared between pulp cells with or without pre-treatment of 5-Aza. Changes in muscle regeneration in response to green fluorescent protein (GFP)-labelled myogenic pulp cell injection in vivo were evaluated using a cardiotoxin (CTX)-induced muscle injury model of the gastrocnemius as well as the masseter muscle in mice.RESULTS:
Pre-treatment of 5-Aza in pulp cells stimulated myotube formation, myogenic differentiation in terms of desmin and myogenin expression, and the level of collagen gel contraction. The local injection of 5-Aza pre-treated myogenic pulp cells was engrafted into the host tissue and indicated signs of enhanced muscle regeneration in both the gastrocnemius and the masseter muscles.CONCLUSION:
The epigenetic modification of pulp cells from extracted premolars and the local injection of myogenic pulp cells may stimulate craniofacial muscles regeneration in vivo.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Regeneración
/
Azacitidina
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Diferenciación Celular
/
Desarrollo de Músculos
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Pulpa Dental
/
Músculo Masetero
Límite:
Adolescent
/
Adult
/
Humans
Idioma:
En
Revista:
Orthod Craniofac Res
Asunto de la revista:
ODONTOLOGIA
/
ORTODONTIA
Año:
2017
Tipo del documento:
Article