Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types.
Genome Biol
; 18(1): 18, 2017 01 26.
Article
en En
| MEDLINE
| ID: mdl-28126036
ABSTRACT
BACKGROUND:
A healthy immune system requires immune cells that adapt rapidly to environmental challenges. This phenotypic plasticity can be mediated by transcriptional and epigenetic variability.RESULTS:
We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome-wide across CD14+CD16- monocytes, CD66b+CD16+ neutrophils, and CD4+CD45RA+ naïve T cells from the same 125 healthy individuals. We discover substantially increased variability in neutrophils compared to monocytes and T cells. In neutrophils, genes with hypervariable expression are found to be implicated in key immune pathways and are associated with cellular properties and environmental exposure. We also observe increased sex-specific gene expression differences in neutrophils. Neutrophil-specific DNA methylation hypervariable sites are enriched at dynamic chromatin regions and active enhancers.CONCLUSIONS:
Our data highlight the importance of transcriptional and epigenetic variability for the key role of neutrophils as the first responders to inflammatory stimuli. We provide a resource to enable further functional studies into the plasticity of immune cells, which can be accessed from http//blueprint-dev.bioinfo.cnio.es/WP10/hypervariability .Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Regulación de la Expresión Génica
/
Epigénesis Genética
/
Estudio de Asociación del Genoma Completo
/
Sistema Inmunológico
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Genome Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
España