Tissue-resident macrophages can contain replication-competent virus in antiretroviral-naive, SIV-infected Asian macaques.
JCI Insight
; 2(4): e91214, 2017 02 23.
Article
en En
| MEDLINE
| ID: mdl-28239657
ABSTRACT
SIV DNA can be detected in lymphoid tissue-resident macrophages of chronically SIV-infected Asian macaques. These macrophages also contain evidence of recently phagocytosed SIV-infected CD4+ T cells. Here, we examine whether these macrophages contain replication-competent virus, whether viral DNA can be detected in tissue-resident macrophages from antiretroviral (ARV) therapy-treated animals and humans, and how the viral sequences amplified from macrophages and contemporaneous CD4+ T cells compare. In ARV-naive animals, we find that lymphoid tissue-resident macrophages contain replication-competent virus if they also contain viral DNA in ARV-naive Asian macaques. The genetic sequence of the virus within these macrophages is similar to those within CD4+ T cells from the same anatomic sites. In ARV-treated animals, we find that viral DNA can be amplified from lymphoid tissue-resident macrophages of SIV-infected Asian macaques that were treated with ARVs for at least 5 months, but we could not detect replication-competent virus from macrophages of animals treated with ARVs. Finally, we could not detect viral DNA in alveolar macrophages from HIV-infected individuals who received ARVs for 3 years and had undetectable viral loads. These data demonstrate that macrophages can contain replication-competent virus, but may not represent a significant reservoir for HIV in vivo.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Replicación Viral
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ADN Viral
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Infecciones por VIH
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Síndrome de Inmunodeficiencia Adquirida del Simio
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VIH-1
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Virus de la Inmunodeficiencia de los Simios
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Macrófagos
Límite:
Animals
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Humans
Idioma:
En
Revista:
JCI Insight
Año:
2017
Tipo del documento:
Article