[Preparation and identification of the polyclonal antibody against ATRX-C2193-2492].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 33(4): 536-539, 2017 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-28395727
ABSTRACT
Objective To prepare the polyclonal antibody against human alpha thalassemia/mental retardation syndrome X-linked (ATRX) C-terminal and study the distribution and expression of ATRX protein in human cervical cancer tissues. Methods The antiserum was obtained from the BALB/c mice immunized with 6His-ATRX-C2193-2492 protein and then purified by the saturated ammonium sulfate precipitation and affinity chromatography. The titer of anti-ATRX polyclonal antibody was determined by ELISA. Its specificity was identified by SDS-PAGE analysis and Western blotting. The expression and location of ATRX in human cervical tissues were analyzed by immunohistochemistry. Results The titer of the polyclonal antibody against 6His-ATRX-C2193-2492 protein was about 112 800. The antibody could recognize 6His-ATRX-C2193-2492 protein specifically. With the polyclonal antibody, the target protein was found mainly in the nucleus of para-carcinoma tissues, and it was also expressed in the nucleus of cervical cancer tissue cells, but the expression in the latter was obviously lower. Conclusion The polyclonal antibody against 6His-ATRX-C2193-2492 protein has been produced successfully and used to detect ATRX protein in human cervical cancer tissues.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas Nucleares
/
Talasemia alfa
/
ADN Helicasas
/
Discapacidad Intelectual Ligada al Cromosoma X
/
Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China