Cell Origin Dictates Programming of Resident versus Recruited Macrophages during Acute Lung Injury.
Am J Respir Cell Mol Biol
; 57(3): 294-306, 2017 09.
Article
en En
| MEDLINE
| ID: mdl-28421818
ABSTRACT
Two populations of alveolar macrophages (AMs) coexist in the inflamed lung resident AMs that arise during embryogenesis, and recruited AMs that originate postnatally from circulating monocytes. The objective of this study was to determine whether origin or environment dictates the transcriptional, metabolic, and functional programming of these two ontologically distinct populations over the time course of acute inflammation. RNA sequencing demonstrated marked transcriptional differences between resident and recruited AMs affecting three main areas proliferation, inflammatory signaling, and metabolism. Functional assays and metabolomic studies confirmed these differences and demonstrated that resident AMs proliferate locally and are governed by increased tricarboxylic acid cycle and amino acid metabolism. Conversely, recruited AMs produce inflammatory cytokines in association with increased glycolytic and arginine metabolism. Collectively, the data show that even though they coexist in the same environment, inflammatory macrophage subsets have distinct immunometabolic programs and perform specialized functions during inflammation that are associated with their cellular origin.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Lesión Pulmonar Aguda
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Am J Respir Cell Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article