Development of a real-time recombinase polymerase amplification assay for rapid and sensitive detection of porcine circovirus 2.
Arch Virol
; 162(8): 2293-2296, 2017 Aug.
Article
en En
| MEDLINE
| ID: mdl-28429132
ABSTRACT
Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was developed to detect PCV-2 using primers and an exo probe specific for the ORF2 gene. The reaction process can be completed in 20 min at 38 °C. The assay only detects PCV-2, as there was no cross-reaction with other pathogens important in pigs. Using the PCV-2 genomic DNA as template, the analytical sensitivity of the real-time RPA was 103 copies. The assay performance was evaluated by testing 38 field samples and compared with real-time PCR. The two assays demonstrated a 100% diagnostic agreement, and PCV-2 DNA was detected in 26 samples. The R2 value of real-time RPA and real-time PCR was 0.954 by linear regression analysis. The real-time RPA assay provides an alternative tool for rapid, simple, and reliable detection of PCV-2, especially in remote and rural areas.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Porcinos
/
Enfermedades de los Porcinos
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Circovirus
/
Infecciones por Circoviridae
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Técnicas de Amplificación de Ácido Nucleico
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
China