Transient cell-specific EXO70A1 activity in the CASP domain and Casparian strip localization.
Nat Plants
; 3: 17058, 2017 04 24.
Article
en En
| MEDLINE
| ID: mdl-28436943
ABSTRACT
In a striking case of evolutionary convergence, polarized cell layers with ring-like diffusion barriers have evolved in both plant and animal lineages independently. In plants, ring-like Casparian strips become localized by the CASPARIAN STRIP MEMBRANE DOMAIN PROTEINS (CASPs). The mechanism of this striking localization, however, has remained enigmatic. Here we present a genetic screen aimed at isolating determinants of CASP localization. One of the mutants, lord of the rings 2 (lotr2)/exo70a1, displays dramatic de-localization of CASPs into randomly localized microdomains. EXO70A1 is a subunit of the exocyst complex, a central component of secretion in eukaryotes. Irradiation of EXO70 subunit genes in plants has suggested specialization of this conserved complex. Intriguingly, lotr2/exo70a1 does neither affect secretion of the CASPs, nor that of other membrane proteins in the endodermis, thus separating exocyst activity in localization from a general defect in secretion. Our results establish EXO70A1 as a central player in Casparian strip formation, generating a transient positional information that will be translated into a precisely localized cell wall modification.
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1
Bases de datos:
MEDLINE
Asunto principal:
Pared Celular
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Arabidopsis
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Proteínas de Arabidopsis
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Proteínas de la Membrana
Idioma:
En
Revista:
Nat Plants
Año:
2017
Tipo del documento:
Article
País de afiliación:
Suiza