Robust IR-based detection of stable and fractionally populated G-C+ and A-T Hoogsteen base pairs in duplex DNA.
FEBS Lett
; 591(12): 1770-1784, 2017 06.
Article
en En
| MEDLINE
| ID: mdl-28524232
ABSTRACT
Noncanonical G-C+ and A-T Hoogsteen base pairs can form in duplex DNA and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in DNA duplexes remains challenging due to difficulties in resolving syn versus antipurine bases with X-ray crystallography; and size limitations and line broadening can make them difficult to characterize by NMR spectroscopy. Here, we show how infrared (IR) spectroscopy can identify G-C+ and A-T Hoogsteen base pairs in duplex DNA across a range of different structural contexts. The utility of IR-based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A-T and G-C+ Hoogsteen base pairs in a DNA duplex where severe broadening complicates detection with NMR.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN
/
Modelos Moleculares
/
Emparejamiento Base
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
FEBS Lett
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos