Hydroxylation of Human Type III Collagen Alpha Chain by Recombinant Coexpression with a Viral Prolyl 4-Hydroxylase in Escherichia coli.

Shi, Jingjing; Ma, Xiaoxuan; Gao, Yuan; Fan, Daidi; Zhu, Chenhui; Mi, Yu; Xue, Wenjiao

Shi, Jingjing; Ma, Xiaoxuan; Gao, Yuan; Fan, Daidi; Zhu, Chenhui; Mi, Yu; Xue, Wenjiao.

Afiliación

Shi J; Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Ma X; Shanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Gao Y; Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Fan D; Shanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Zhu C; Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Mi Y; Shanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China.
Xue W; Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an, 710069, Shaanxi, China. fandaidi@nwu.edu.cn.

Protein J ; 36(4): 322-331, 2017 08.

Article en En | MEDLINE | ID: mdl-28589291

Asunto(s)

Colágeno Tipo III/metabolismo; Escherichia coli/metabolismo; Plásmidos/metabolismo; Prolil Hidroxilasas/metabolismo; Proteínas Recombinantes/metabolismo; Proteínas Virales/metabolismo; Secuencia de Aminoácidos; Animales; Línea Celular; Proliferación Celular/efectos de los fármacos; Colágeno Tipo III/química; Colágeno Tipo III/genética; Colágeno Tipo III/farmacología; Cricetinae; Escherichia coli/genética; Expresión Génica; Humanos; Hidroxilación; Phycodnaviridae/química; Phycodnaviridae/enzimología; Plásmidos/química; Prolil Hidroxilasas/genética; Regiones Promotoras Genéticas; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/farmacología; Alineación de Secuencia; Transformación Bacteriana; Proteínas Virales/genética

Palabras clave

Coexpression; Escherichia coli; Human collagen α1(III) chain; Hydroxylation; Viral prolyl 4-hydroxylase

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Plásmidos / Proteínas Virales / Proteínas Recombinantes / Colágeno Tipo III / Escherichia coli / Prolil Hidroxilasas Límite: Animals / Humans Idioma: En Revista: Protein J Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: China

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