Time course of Tau toxicity and pharmacologic prevention in a cell model of Tauopathy.
Neurobiol Aging
; 57: 47-63, 2017 09.
Article
en En
| MEDLINE
| ID: mdl-28600952
ABSTRACT
The aggregation of Tau protein is a hallmark of neurodegenerative diseases including Alzheimer's disease. Previously, we generated a cell model of tauopathy based on the 4-repeat domain with the FTDP-17 mutation ΔK280 (Tau4RDΔK) which is expressed in a regulatable fashion (tet-on). The deletion variant ΔK280 is highly amyloidogenic and forms fibrous aggregates in neuroblastoma N2a cells staining with the reporter dye Thioflavin S. The aggregation of Tau4RDΔK is toxic, contrary to wildtype or anti-aggregant variants of the protein. Using a novel approach for monitoring in situ Tau aggregation and toxicity by combination of microscopic analysis with FACS and biochemical analysis of cells enabled the dissection of the aggregating species which cause a time-dependent increase of toxicity. The dominant initiating step is the dimerization of Tau4RDΔK which leads to further aggregation and induces a strong increase in reactive oxygen species (ROS) and cytoplasmic Ca2+ which damage the membranes and cause cell death. Tau-based treatments using Tau aggregation inhibitors reduce both soluble oligomeric and fully aggregated Tau species and decrease their toxicity.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Tiazoles
/
Proteínas tau
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Tauopatías
/
Hidrazinas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Neurobiol Aging
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania