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Loss of prion protein induces a primed state of type I interferon-responsive genes.
Malachin, Giulia; Reiten, Malin R; Salvesen, Øyvind; Aanes, Håvard; Kamstra, Jorke H; Skovgaard, Kerstin; Heegaard, Peter M H; Ersdal, Cecilie; Espenes, Arild; Tranulis, Michael A; Bakkebø, Maren K.
Afiliación
  • Malachin G; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Reiten MR; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Salvesen Ø; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Aanes H; Department of Microbiology, Division of diagnostics and intervention, Institute of Clinical Medicine, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
  • Kamstra JH; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Skovgaard K; Innate Immunology Group, Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Heegaard PMH; Innate Immunology Group, Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • Ersdal C; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Espenes A; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Tranulis MA; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
  • Bakkebø MK; Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway.
PLoS One ; 12(6): e0179881, 2017.
Article en En | MEDLINE | ID: mdl-28651013
The cellular prion protein (PrPC) has been extensively studied because of its pivotal role in prion diseases; however, its functions remain incompletely understood. A unique line of goats has been identified that carries a nonsense mutation that abolishes synthesis of PrPC. In these animals, the PrP-encoding mRNA is rapidly degraded. Goats without PrPC are valuable in re-addressing loss-of-function phenotypes observed in Prnp knockout mice. As PrPC has been ascribed various roles in immune cells, we analyzed transcriptomic responses to loss of PrPC in peripheral blood mononuclear cells (PBMCs) from normal goat kids (n = 8, PRNP+/+) and goat kids without PrPC (n = 8, PRNPTer/Ter) by mRNA sequencing. PBMCs normally express moderate levels of PrPC. The vast majority of genes were similarly expressed in the two groups. However, a curated list of 86 differentially expressed genes delineated the two genotypes. About 70% of these were classified as interferon-responsive genes. In goats without PrPC, the majority of type I interferon-responsive genes were in a primed, modestly upregulated state, with fold changes ranging from 1.4 to 3.7. Among these were ISG15, DDX58 (RIG-1), MX1, MX2, OAS1, OAS2 and DRAM1, all of which have important roles in pathogen defense, cell proliferation, apoptosis, immunomodulation and DNA damage response. Our data suggest that PrPC contributes to the fine-tuning of resting state PBMCs expression level of type I interferon-responsive genes. The molecular mechanism by which this is achieved will be an important topic for further research into PrPC physiology.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Cabras / Interferón Tipo I / Proteínas PrPC Límite: Animals / Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Noruega

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Cabras / Interferón Tipo I / Proteínas PrPC Límite: Animals / Female / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Noruega