Identification of genes dysregulated by elevation of microRNA-210 levels in human trophoblasts cell line, Swan 71.
Am J Reprod Immunol
; 78(5)2017 Nov.
Article
en En
| MEDLINE
| ID: mdl-28653360
ABSTRACT
PROBLEM:
Preeclampsia is a serious pregnancy disorder characterized by gestational hypertension and proteinuria. miR-210 is significantly overexpressed in the placentas of preeclampsia patients. METHOD OF STUDY Swan 71 cells, first-trimester human trophoblastic cell line, were transfected with hsa-miR-210-3p oligonucleotides by electroporation. Altered transcriptome was analyzed using microarray technique. Differentially expressed genes (DEGs) were clustered into Gene Ontology annotation biological processes. The extent of physical interaction between miR-210 and IGFBP3 mRNA was assessed via ribonucleoprotein immunoprecipitation.RESULTS:
Microarray analysis showed 408 DEGs by elevated levels of miR-210 in Swan 71 cells. These genes were enriched in several biological processes involved in the pathogenesis of preeclampsia. IGFBP3, a gene associated with preeclampsia pathophysiology, was validated as a target gene of miR-210.CONCLUSION:
We have demonstrated that elevated miR-210 levels in human trophoblast alter the expression profile of known preeclampsia-associated genes, and of gene targets involved in various biological processes essential to preeclampsia progression.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Placenta
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Preeclampsia
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Trofoblastos
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Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina
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MicroARNs
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2017
Tipo del documento:
Article