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Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses.
Coppiello, Giulia; Abizanda, Gloria; Aguado, Natalia; Iglesias, Elena; Arellano-Viera, Estibaliz; Rodriguez-Madoz, Juan R; Carvajal-Vergara, Xonia; Prosper, Felipe; Aranguren, Xabier L.
Afiliación
  • Coppiello G; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Abizanda G; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Aguado N; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Iglesias E; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Arellano-Viera E; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Rodriguez-Madoz JR; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Carvajal-Vergara X; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Prosper F; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: fprospe
  • Aranguren XL; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: xlaranguren@unav.es.
Stem Cell Res ; 21: 1-4, 2017 05.
Article en En | MEDLINE | ID: mdl-28677526
ABSTRACT
We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC. Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts. Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piel / Factores de Transcripción / Transducción Genética / Virus Sendai / Células Madre Pluripotentes Inducidas / Fibroblastos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: España
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Piel / Factores de Transcripción / Transducción Genética / Virus Sendai / Células Madre Pluripotentes Inducidas / Fibroblastos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: España