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In vivo multiphoton imaging of a diverse array of fluorophores to investigate deep neurovascular structure.
Miller, David R; Hassan, Ahmed M; Jarrett, Jeremy W; Medina, Flor A; Perillo, Evan P; Hagan, Kristen; Shams Kazmi, S M; Clark, Taylor A; Sullender, Colin T; Jones, Theresa A; Zemelman, Boris V; Dunn, Andrew K.
Afiliación
  • Miller DR; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Hassan AM; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Jarrett JW; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Medina FA; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Perillo EP; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Hagan K; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Shams Kazmi SM; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Clark TA; Department of Neuroscience, The University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA.
  • Sullender CT; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
  • Jones TA; Department of Neuroscience, The University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA.
  • Zemelman BV; Department of Neuroscience, The University of Texas at Austin, 2415 Speedway, Austin, TX 78712, USA.
  • Dunn AK; Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.
Biomed Opt Express ; 8(7): 3470-3481, 2017 Jul 01.
Article en En | MEDLINE | ID: mdl-28717582
ABSTRACT
We perform high-resolution, non-invasive, in vivo deep-tissue imaging of the mouse neocortex using multiphoton microscopy with a high repetition rate optical parametric amplifier laser source tunable between λ=1,100 and 1,400 nm. By combining the high repetition rate (511 kHz) and high pulse energy (400 nJ) of our amplifier laser system, we demonstrate imaging of vasculature labeled with Texas Red and Indocyanine Green, and neurons expressing tdTomato and yellow fluorescent protein. We measure the blood flow speed of a single capillary at a depth of 1.2 mm, and image vasculature to a depth of 1.53 mm with fine axial steps (5 µm) and reasonable acquisition times. The high image quality enabled analysis of vascular morphology at depths to 1.45 mm.
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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos