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Preparation of intact mitochondria using free-flow isoelectric focusing with post-pH gradient sample injection for morphological, functional and proteomics studies.
He, Yu-Chen; Kong, Fan-Zhi; Fan, Liu-Yin; Wu, Jane Y; Liu, Xiao-Ping; Li, Jun; Sun, Yan; Zhang, Qiang; Yang, Ying; Wu, Xue-Jing; Xiao, Hua; Cao, Cheng-Xi.
Afiliación
  • He YC; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Kong FZ; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Fan LY; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Wu JY; Department of Neurology, Lurie Cancer Center, Center for Genetic Medicine, Northwestern University, Chicago, IL 60611-3010, USA.
  • Liu XP; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Li J; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Sun Y; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Zhang Q; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Yang Y; School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
  • Wu XJ; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Xiao H; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: huaxiao@sjtu.edu.cn.
  • Cao CX; State Key Laboratory of Microbial Metabolism, Laboratory of Analytical Biochemistry and Bioseparation, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address: cxcao@sjtu.edu.cn.
Anal Chim Acta ; 982: 200-208, 2017 Aug 22.
Article en En | MEDLINE | ID: mdl-28734361
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Mitocondrias Hepáticas / Proteínas Mitocondriales / Proteómica / Focalización Isoeléctrica Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2017 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Mitocondrias Hepáticas / Proteínas Mitocondriales / Proteómica / Focalización Isoeléctrica Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2017 Tipo del documento: Article País de afiliación: China