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Conformational landscape of a virus by single-particle X-ray scattering.
Hosseinizadeh, Ahmad; Mashayekhi, Ghoncheh; Copperman, Jeremy; Schwander, Peter; Dashti, Ali; Sepehr, Reyhaneh; Fung, Russell; Schmidt, Marius; Yoon, Chun Hong; Hogue, Brenda G; Williams, Garth J; Aquila, Andrew; Ourmazd, Abbas.
Afiliación
  • Hosseinizadeh A; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Mashayekhi G; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Copperman J; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Schwander P; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Dashti A; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Sepehr R; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Fung R; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Schmidt M; Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
  • Yoon CH; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Hogue BG; Biodesign Center for Immunotherapy, Vaccines and Virotherapy, Arizona State University, Tempe, Arizona, USA.
  • Williams GJ; Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, Arizona, USA.
  • Aquila A; School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
  • Ourmazd A; Brookhaven National Laboratory, Upton, New York, USA.
Nat Methods ; 14(9): 877-881, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28805793
ABSTRACT
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. These results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bacteriófagos / Algoritmos / ADN Viral / Cristalografía por Rayos X / Imagenología Tridimensional / Dispersión del Ángulo Pequeño Tipo de estudio: Diagnostic_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Bacteriófagos / Algoritmos / ADN Viral / Cristalografía por Rayos X / Imagenología Tridimensional / Dispersión del Ángulo Pequeño Tipo de estudio: Diagnostic_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos