Conformational landscape of a virus by single-particle X-ray scattering.
Nat Methods
; 14(9): 877-881, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28805793
ABSTRACT
Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. These results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Algoritmos
/
ADN Viral
/
Cristalografía por Rayos X
/
Imagenología Tridimensional
/
Dispersión del Ángulo Pequeño
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos