A rational approach to enhancing antibody Fc homodimer formation for robust production of antibody mixture in a single cell line.

Yu, Jie; Wang, Xiaoxiao; Xu, Tao; Jin, Qiuheng; Duan, Jinyuan; Wu, Jie; Wu, Haiyan; Xu, Ting; Ye, Sheng

Yu, Jie; Wang, Xiaoxiao; Xu, Tao; Jin, Qiuheng; Duan, Jinyuan; Wu, Jie; Wu, Haiyan; Xu, Ting; Ye, Sheng.

Afiliación

Yu J; From the Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang 310058 and.
Wang X; Alphamab Co. Ltd., Suzhou 215125, China.
Xu T; Alphamab Co. Ltd., Suzhou 215125, China.
Jin Q; From the Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang 310058 and.
Duan J; From the Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang 310058 and.
Wu J; Alphamab Co. Ltd., Suzhou 215125, China.
Wu H; Alphamab Co. Ltd., Suzhou 215125, China.
Xu T; Alphamab Co. Ltd., Suzhou 215125, China tingxu@alphamab.com.
Ye S; From the Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang 310058 and sye@zju.edu.cn.

J Biol Chem ; 292(43): 17885-17896, 2017 10 27.

Article en En | MEDLINE | ID: mdl-28878018

Asunto(s)

Fragmentos Fc de Inmunoglobulinas/química; Multimerización de Proteína; Células HEK293; Humanos; Fragmentos Fc de Inmunoglobulinas/genética; Fragmentos Fc de Inmunoglobulinas/metabolismo; Dominios Proteicos

Palabras clave

antibody engineering; crystal structure; epidermal growth factor receptor (EGFR); monoclonal antibody; protein engineering; structure based protein engineering

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Fragmentos Fc de Inmunoglobulinas / Multimerización de Proteína Límite: Humans Idioma: En Revista: J Biol Chem Año: 2017 Tipo del documento: Article

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