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Ultra-Sensitive NT-proBNP Quantification for Early Detection of Risk Factors Leading to Heart Failure.
Song, Keum-Soo; Nimse, Satish Balasaheb; Sonawane, Mukesh Digambar; Warkad, Shrikant Dashrath; Kim, Taisun.
Afiliación
  • Song KS; Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. hanlimsk@empal.com.
  • Nimse SB; Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. satish_nimse@hallym.ac.kr.
  • Sonawane MD; Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. mukeshsonawane87@hallym.ac.kr.
  • Warkad SD; Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. shrikant.warkad@hallym.ac.kr.
  • Kim T; Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea. tskim@hallym.ac.kr.
Sensors (Basel) ; 17(9)2017 Sep 14.
Article en En | MEDLINE | ID: mdl-28906476
Cardiovascular diseases such as acute myocardial infarction and heart failure accounted for the death of 17.5 million people (31% of all global deaths) in 2015. Monitoring the level of circulating N-terminal proBNP (NT-proBNP) is crucial for the detection of people at risk of heart failure. In this article, we describe a novel ultra-sensitive NT-proBNP test (us-NT-proBNP) that allows the quantification of circulating NT-proBNP in 30 min at 25 °C in the linear detection range of 7.0-600 pg/mL. It is a first report on the application of a fluorescence bead labeled detection antibody, DNA-guided detection method, and glass fiber membrane platform for the quantification of NT-proBNP in clinical samples. Limit of blank, limit of detection, and limit of quantification were 2.0 pg/mL, 3.7 pg/mL, and 7 pg/mL, respectively. The coefficient of variation was found to be less than 10% in the entire detection range of 7-600 pg/mL. The test demonstrated specificity for NT-proBNP without interferences from bilirubin, intra-lipid, biotin, and hemoglobin. The serial dilution test for plasma samples containing various NT-proBNP levels showed the linear decrement in concentration with the regression coefficient of 0.980-0.998. These results indicate that us-NT-proBNP test does not suffer from the interference of the plasma components for the measurement of NT-proBNP in clinical samples.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Insuficiencia Cardíaca Tipo de estudio: Diagnostic_studies / Etiology_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2017 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Insuficiencia Cardíaca Tipo de estudio: Diagnostic_studies / Etiology_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Sensors (Basel) Año: 2017 Tipo del documento: Article