Your browser doesn't support javascript.
loading
Pluripotent stem cell differentiation reveals distinct developmental pathways regulating lung- versus thyroid-lineage specification.
Serra, Maria; Alysandratos, Konstantinos-Dionysios; Hawkins, Finn; McCauley, Katherine B; Jacob, Anjali; Choi, Jinyoung; Caballero, Ignacio S; Vedaie, Marall; Kurmann, Anita A; Ikonomou, Laertis; Hollenberg, Anthony N; Shannon, John M; Kotton, Darrell N.
Afiliación
  • Serra M; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • Alysandratos KD; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
  • Hawkins F; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • McCauley KB; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
  • Jacob A; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • Choi J; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
  • Caballero IS; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • Vedaie M; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
  • Kurmann AA; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • Ikonomou L; The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
  • Hollenberg AN; Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston MA 02215, USA.
  • Shannon JM; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
  • Kotton DN; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
Development ; 144(21): 3879-3893, 2017 11 01.
Article en En | MEDLINE | ID: mdl-28947536
The in vitro-directed differentiation of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. However, there has been significant uncertainty regarding a method to separately derive lung versus thyroid epithelial lineages, as these two cell types each originate from Nkx2-1+ foregut progenitors and the minimal pathways claimed to regulate their distinct lineage specification in vivo or in vitro have varied in previous reports. Here, we employ PSCs to identify the key minimal signaling pathways (Wnt+BMP versus BMP+FGF) that regulate distinct lung- versus thyroid-lineage specification, respectively, from foregut endoderm. In contrast to most previous reports, these minimal pathways appear to be evolutionarily conserved between mice and humans, and FGF signaling, although required for thyroid specification, unexpectedly appears to be dispensable for lung specification. Once specified, distinct Nkx2-1+ lung or thyroid progenitor pools can now be independently derived for functional 3D culture maturation, basic developmental studies or future regenerative therapies.
Asunto(s)
Palabras clave

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glándula Tiroides / Transducción de Señal / Diferenciación Celular / Tipificación del Cuerpo / Células Madre Pluripotentes / Pulmón Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Glándula Tiroides / Transducción de Señal / Diferenciación Celular / Tipificación del Cuerpo / Células Madre Pluripotentes / Pulmón Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos