Arctigenin represses TGF-ß-induced epithelial mesenchymal transition in human lung cancer cells.

ABSTRACT

Arctigenin (ARC) is a lignan that is abundant in Asteraceae plants, which show anti-inflammatory and anti-cancer activities. The current study investigated whether ARC affects cancer progression and metastasis, focusing on EMT using invasive human non-small cell lung cancer (NSCLC) cells. No toxicity was observed in the cells treated with different doses of ARC (12-100 µM). The treatment of ARC repressed TGF-ß-stimulated changes of metastatic morphology and cell invasion and migration. ARC inhibited TGF-ß-induced phosphorylation and transcriptional activity of smad2/3, and expression of snail. ARC also decreased expression of N-cadherin and increased expression of E-cadherin in dose-dependent and time-dependent manners. These changes were accompanied by decreased amount of phospho-smad2/3 in nucleus and nuclear translocation of smad2/3. Moreover, ARC repressed TGF-ß-induced phosphorylation of ERK and transcriptional activity of ß-catenin. Our data demonstrate anti-metastatic activity of ARC in lung cancer model.