Isolation of myogenic progenitor cell population from human placenta: A pilot study.
J Pediatr Surg
; 52(12): 2078-2082, 2017 Dec.
Article
en En
| MEDLINE
| ID: mdl-28964407
ABSTRACT
PURPOSE:
The purpose of this study was to demonstrate a method of isolating myogenic progenitor cells from human placenta chorionic villi and to confirm the myogenic characteristics of the isolated cells.METHODS:
Cells were isolated from chorionic villi of a second trimester male placenta via a combined enzymatic digestion and explant culture. A morphologically distinct subpopulation of elongated and multinucleated cells was identified. This subpopulation was manually passaged from the explant culture, expanded, and analyzed by fluorescence in situ hybridization (FISH) assay, immunocytochemistry, and flow cytometry. Myogenic characteristics including alignment and fusion were tested by growing these cells on aligned polylactic acid microfibrous scaffold in a fusion media composed of 2% horse serum in Dulbecco's modified Eagle medium/high glucose.RESULTS:
The expanded subpopulation was uniformly positive for integrin α-7. Presence of Y-chromosome by FISH analysis confirmed chorionic villus origin rather than maternal cell contamination. Isolated cells grew, aligned, and fused on the microfibrous scaffold, and they expressed myogenin, desmin, and MHC confirming their myogenic identity.CONCLUSION:
Myogenic progenitor cells can be isolated from human chorionic villi. This opens the possibility for translational and clinical applications using autologous myogenic cells for possible engraftment in treatment of chest and abdominal wall defects.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Placenta
/
Células Madre
/
Vellosidades Coriónicas
Tipo de estudio:
Prognostic_studies
Límite:
Female
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Humans
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Male
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Pregnancy
Idioma:
En
Revista:
J Pediatr Surg
Año:
2017
Tipo del documento:
Article