Letrozole induced low estrogen levels affected the expressions of duodenal and renal calcium-processing gene in laying hens.
Gen Comp Endocrinol
; 255: 49-55, 2018 01 01.
Article
en En
| MEDLINE
| ID: mdl-29037849
ABSTRACT
Estrogen regulates the calcium homeostasis in hens, but the mechanisms involved are still unclear fully. In this study, we investigated whether letrozole (LZ) induced low estrogen levels affected the calcium absorption and transport in layers. In the duodenum, we observed a significant decrease of mRNA expressions of Calbindin-28k (CaBP-28k) and plasma membrane Ca2+-ATPase (PMCA 1b) while CaBP-28k protein expression was declined in birds with LZ treatment, and the mRNA levels of duodenal transient receptor potential vanilloid 6 (TRPV6) and Na+/Ca2+ exchanger 1 (NCX1) were not affected. Interestingly, we observed the different changes in the kidney. The renal mRNA expressions of TRPV6 and NCX1 were unregulated while the PMCA1b was down-regulated in low estrogen layers, however, the CaBP-28k gene and protein expressions were no changed in the kidney. Furthermore, it showed that the duodenal estradiol receptor 2 (ESR2) transcripts rather than parathyroid hormone 1 receptor (PTH1R) and calcitonin receptor (CALCR) played key roles to down-regulate calcium transport in LZ-treated birds. In conclusion, CaBP-28k, PMCA 1b and ESR2 genes in the duodenum may be primary targets for estrogen regulation in order to control calcium homeostasis in hens.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Triazoles
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Pollos
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Regulación de la Expresión Génica
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Calcio
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Duodeno
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Estrógenos
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Riñón
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Nitrilos
Límite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Año:
2018
Tipo del documento:
Article
País de afiliación:
China