Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq.
Methods Mol Biol
; 1673: 177-192, 2018.
Article
en En
| MEDLINE
| ID: mdl-29130173
ABSTRACT
RNA sequencing (RNAseq) enables transcriptional profiling of many organisms. This chapter describes the use of RNAseq in prokaryotes to identify quorum sensing (QS)-controlled transcripts by comparing samples from QS-induced and -uninduced conditions. Briefly, each RNA sample is converted to ds-cDNA in a method that limits amplification of ribosomal RNA species. The ds-cDNA contains adapters that enable sequencing and quantification by next-generation sequencing (NGS).
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Regulación Bacteriana de la Expresión Génica
/
Análisis de Secuencia de ARN
/
Perfilación de la Expresión Génica
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Percepción de Quorum
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos