Divergent susceptibilities to AAV-SaCas9-gRNA vector-mediated genome-editing in a single-cell-derived cell population.

Morsy, Salma G; Tonne, Jason M; Zhu, Yaxi; Lu, Brian; Budzik, Karol; Krempski, James W; Ali, Sherine A; El-Feky, Mohamed A; Ikeda, Yasuhiro

Morsy, Salma G; Tonne, Jason M; Zhu, Yaxi; Lu, Brian; Budzik, Karol; Krempski, James W; Ali, Sherine A; El-Feky, Mohamed A; Ikeda, Yasuhiro.

Afiliación

Morsy SG; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
Tonne JM; Cancer Biology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
Zhu Y; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
Lu B; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
Budzik K; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
Krempski JW; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
Ali SA; Department of Molecular Medicine, Mayo Clinic, College of Medicine, 200 First St. SW, Rochester, MN, 55905, USA.
El-Feky MA; Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Ikeda Y; Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.

BMC Res Notes ; 10(1): 720, 2017 Dec 08.

Article en En | MEDLINE | ID: mdl-29221488

Asunto(s)

Proteínas Asociadas a CRISPR/genética; Sistemas CRISPR-Cas; Dependovirus; Endonucleasas/genética; Secuenciación del Exoma; Edición Génica; Vectores Genéticos; ARN Guía de Kinetoplastida; Staphylococcus aureus/enzimología; Proteínas Bacterianas; Proteína 9 Asociada a CRISPR; Línea Celular; Susceptibilidad a Enfermedades; Proteínas Fluorescentes Verdes; Células HEK293; Humanos

Palabras clave

Adeno Associated viral vector; Genome editing resistance; Off-target; SaCas9 optimization; Whole exome sequencing

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Staphylococcus aureus / ARN Guía de Kinetoplastida / Dependovirus / Endonucleasas / Proteínas Asociadas a CRISPR / Sistemas CRISPR-Cas / Edición Génica / Secuenciación del Exoma / Vectores Genéticos Límite: Humans Idioma: En Revista: BMC Res Notes Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos

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