[Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology].
Fa Yi Xue Za Zhi
; 33(3): 277-280, 2017 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-29230994
ABSTRACT
OBJECTIVES:
To detect the genotype of ABO blood group by SNaPshot technology.METHODS:
DNA were extracted from the peripheral blood samples with known blood groups ï¼obtained by serologyï¼ of 107 unrelated individuals in Yunnan. Six SNP loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions were detected by SNaPshot Multiplex kit, and relevant genetics parameters were calculated.RESULTS:
In 107 blood samples, the allele frequencies of types A, B, OA, and OG were 0.355 1, 0.168 2, 0.230 0 and 0.247 6, respectively, while that of types AG and cis AB were not detected. The genotyping results of ABO blood group were consistent with that of serologic testing.CONCLUSIONS:
SNaPshot technology can be adapted for genotyping of ABO blood group.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo ABO
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
Pueblo Asiatico
/
Genotipo
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China