Immunoreaction-Mediated Aggregation of Gold Nanoparticles for Sensitive and Selective Assay of Hepatitis B Surface Antigen.
J Nanosci Nanotechnol
; 18(2): 1083-1088, 2018 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-29448537
ABSTRACT
A rapid, sensitive and quantitative assay method for Hepatitis B surface antigen (HBsAg) is of paramount importance for the drug development and in the diagnosis of this disease. Here, we proposed a novel biosensor that sensitively and selectively screen Hepatitis B surface antigen. This strategy relies on the cross-linking aggregation of gold nanoparticles (AuNPs) that were decorated with Hepatitis B surface antibody (HBsAb) and Raman reporter 5-thio-nitrobenzoic acid (TNB) by Hepatitis B surface antigen. The immune reaction between HBsAb and HBsAg offers this strategy high specificity, and the use of AuNPs additionally allows a visual and homogeneous assay format, thus permitting improved simplicity and throughput of the assays. The selectivity and sensitivity in HBsAg assay were achieved with a wide linear response range from 0.5 ng/mL to 50 ng/mL and a detection limit of 0.2 ng/mL. The results indicated that this strategy can offer a simple, robust and convenient platform for HBsAg analysis and related biochemical studies with high sensitivity and selectivity.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
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Nanopartículas del Metal
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Oro
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Antígenos de Superficie de la Hepatitis B
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Nanosci Nanotechnol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Vietnam