Identification and characterization of neutralization epitopes at VP2 and VP1 of enterovirus A71.
J Med Virol
; 90(6): 1164-1167, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29457642
ABSTRACT
Enterovirus A71 (EV-A71) neutralization escape mutants were generated with monoclonal antibody MAB979 (Millipore). The VP2-T141I and VP1-D14N substitutions were identified. Using reverse genetics, infectious clones with these substitutions were constructed and tested by neutralization assay with immune sera from mice and humans. The N-terminus VP1-14 is more important than EF loop VP2-141 in acute human infection, mainly because it recognised IgM present in acute infection. The N-terminus VP1 could be a useful target for diagnostics and therapeutic antibodies in acute infection.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Enterovirus Humano A
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Proteínas de la Cápside
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Anticuerpos Neutralizantes
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Anticuerpos Antivirales
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Epítopos
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Animals
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Humans
Idioma:
En
Revista:
J Med Virol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Malasia