Isothermal microcalorimetry - A quantitative method to monitor Trypanosoma congolense growth and growth inhibition by trypanocidal drugs in real time.
Int J Parasitol Drugs Drug Resist
; 8(2): 159-164, 2018 08.
Article
en En
| MEDLINE
| ID: mdl-29587237
ABSTRACT
Trypanosoma congolense is a protozoan parasite that is transmitted by tsetse flies, causing African Animal Trypanosomiasis, also known as Nagana, in sub-Saharan Africa. Nagana is a fatal disease of livestock that causes severe economic losses. Two drugs are available, diminazene and isometamidium, yet successful treatment is jeopardized by drug resistant T. congolense. Isothermal microcalorimetry is a highly sensitive tool that can be used to study growth of the extracellular T. congolense parasites or to study parasite growth inhibition after the addition of antitrypanosomal drugs. Time of drug action and time to kill can be quantified in a simple way by real time heat flow measurements. We established a robust protocol for the microcalorimetric studies of T. congolense and developed mathematical computations in R to calculate different parameters related to growth and the kinetics of drug action. We demonstrate the feasibility and benefit of the method exemplary with the two standard drugs, diminazene aceturate and isometamidium chloride. The method and the mathematical approach can be translated to study other pathogenic or non-pathogenic cells if they are metabolically active and grow under axenic conditions.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Temperatura
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Tripanocidas
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Trypanosoma congolense
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Calorimetría
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Antiprotozoarios
Tipo de estudio:
Diagnostic_studies
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Guideline
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Int J Parasitol Drugs Drug Resist
Año:
2018
Tipo del documento:
Article
País de afiliación:
Suiza