Raman Spectroscopic and Microscopic Analysis for Monitoring Renal Osteodystrophy Signatures.
Biosensors (Basel)
; 8(2)2018 Apr 08.
Article
en En
| MEDLINE
| ID: mdl-29642494
ABSTRACT
Defining the pathogenesis of renal osteodystrophy (ROD) and its treatment efficacy are difficult, since many factors potentially affect bone quality. In this study, confocal Raman microscopy and parallel statistical analysis were used to identify differences in bone composition between healthy and ROD bone tissues through direct visualization of three main compositional parametric ratios, namely, calcium content, mineral-to-matrix, and carbonate-to-matrix. Besides the substantially lower values found in ROD specimens for these representative ratios, an obvious accumulation of phenylalanine is Raman spectroscopically observed for the first time in ROD samples and reported here. Thus, elevated phenylalanine could also be considered as an indicator of the disease. Since the image results are based on tens of thousands of spectra per sample, not only are the average ratios statistically significantly different for normal and ROD bone, but the method is clearly powerful in distinguishing between the two types of samples. Furthermore, the statistical outcomes demonstrate that only a relatively small number of spectra need to be recorded in order to classify the samples. This work thus opens the possibility of future development of in vivo Raman sensors for assessment of bone structure, remodeling, and mineralization, where different biomarkers are simultaneously detected with unprecedented accuracy.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Trastorno Mineral y Óseo Asociado a la Enfermedad Renal Crónica
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Espectrometría Raman
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Microscopía
Tipo de estudio:
Prognostic_studies
Límite:
Aged
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Humans
Idioma:
En
Revista:
Biosensors (Basel)
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos