Design, synthesis, and biological evaluation of 2-substituted-2,3,4,9-tetrahydrospiro-ß-carboline-3-carboxylic acid derivatives as first-in-class mast cell stabilizers.

ABSTRACT

Mast cell degranulation plays a momentous role in myriad diseases like asthma, eczema, allergic rhinitis, and conjunctivitis as well as anaphylactic shock; hence, there is an unmet need for developing new mast cells stabilizers. The reported mast cell stabilizers have a heterocyclic moiety and an acidic group. Furthermore, the role of tryptophan in suppression of mast cell activation is established. Hence, we prepared constrained analogs of tryptophan, which are derivatives of 2,3,4,9-tetrahydrospiro-ß-carboline-3-carboxylic acid, and evaluated them for ex vivo inhibition of compound 48/80-induced mast degranulation activity. By comparing IC50 (µM) values with that of the standard drug sodium cromoglycate (IC50 = 0.489 ± 0.003 µM), compounds with bulky groups like heptyl (compound 9; IC50 = 0.389 ± 0.015 µM) and octyl (compound 10; IC50 = 0.354 ± 0.023 µM) were found to be of similar potency as sodium cromoglycate. Furthermore, the polar group-containing compounds like the chloropropyl (compound 16; IC50 = 0.382 ± 0.083 µM) and benzoyl derivative (compound 14; IC50 = 00.469 ± 0.032 µM) were also found to be of similar potency as sodium cromoglycate. This is a seminal study of spiro-ß-carboline mast cell stabilization having a wider scope in mast cell research; yet, the mechanism of action remains elusive.