Swimming Exercise Induced Reversed Expression of miR-96 and Its Target Gene NaV1.3 in Diabetic Peripheral Neuropathy in Rats.
Chin J Physiol
; 61(2): 124-129, 2018 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-29689688
ABSTRACT
Diabetes is a common metabolic disease which leads to diabetic peripheral neuropathy. Recently, the role of microRNA-96 (miR-96) in alleviating neuropathic pain by inhibiting the expression of NaV1.3, an isoform of voltage-gated sodium channels, has been shown. Peripheral nerve injuries result in NaV1.3 elevation. Exercise has beneficial role in diabetes management and peripheral neuropathy. However, the effects of exercise on miR-96 and its target gene NaV1.3 in diabetic rats are unknown. Therefore, the present study investigated the effects of exercise training on the expression of miR-96 and NaV1.3 in diabetic rats. For this purpose, rats were randomly divided into four groups control, exercise, diabetic and diabetic-exercise groups. Type 2 diabetes was induced by a high-fat diet and the administration of streptozotocin (STZ) (35 mg/kg, i.p.). The exercise groups were subjected to swimming exercise 5 days/week for 10 weeks. At the end of the treatment period, thermal pain threshold, determined through the tail-flick test, and the expression levels of miR-96 and its target gene NaV1.3 were determined by reverse transcription (RT)-PCR in the sciatic nerve tissues of the rats. Data of the present study indicated that diabetes diminished miR-96 expression levels, but significantly upregulated NaV1.3 expression in the sciatic nerve. On exercise training, miR-96 expression was reversed with concurrent down-regulation of the NaV1.3 expression. This study introduced a new and potential miRNA-dependent mechanism for exerciseinduced protective effects against diabetic thermal hyperalgesia.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Nervio Ciático
/
Natación
/
MicroARNs
/
Diabetes Mellitus Experimental
/
Diabetes Mellitus Tipo 2
/
Neuropatías Diabéticas
/
Terapia por Ejercicio
/
Canal de Sodio Activado por Voltaje NAV1.3
Límite:
Animals
Idioma:
En
Revista:
Chin J Physiol
Año:
2018
Tipo del documento:
Article
País de afiliación:
Irán