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Metabolic engineering of Pichia pastoris.
Peña, David A; Gasser, Brigitte; Zanghellini, Jürgen; Steiger, Matthias G; Mattanovich, Diethard.
Afiliación
  • Peña DA; Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
  • Gasser B; Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria; CD-Laboratory for Growth-Decoupled Protein Production in Yeast, Department of Biotechnology, University of Natural Resources and Life Sc
  • Zanghellini J; Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
  • Steiger MG; Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria.
  • Mattanovich D; Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Vienna, Austria. Electronic address: diethard.mattanovich@boku.ac.at.
Metab Eng ; 50: 2-15, 2018 11.
Article en En | MEDLINE | ID: mdl-29704654
Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin. We summarize here the impressive methodological developments of the last years to model and analyze the metabolism of P. pastoris, and to engineer its genome and metabolic pathways. Efficient methods to insert, modify or delete genes via homologous recombination and CRISPR/Cas9, supported by modular cloning techniques, have been reported. An outstanding early example of metabolic engineering in P. pastoris was the humanization of protein glycosylation. More recently the cell metabolism was engineered also to enhance the productivity of heterologous proteins. The last few years have seen an increased number of metabolic pathway design and engineering in P. pastoris, mainly towards the production of complex (secondary) metabolites. In this review, we discuss the potential role of P. pastoris as a platform for metabolic engineering, its strengths, and major requirements for future developments of chassis strains based on synthetic biology principles.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pichia / Ingeniería Metabólica / Sistemas CRISPR-Cas Idioma: En Revista: Metab Eng Asunto de la revista: ENGENHARIA BIOMEDICA / METABOLISMO Año: 2018 Tipo del documento: Article País de afiliación: Austria

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pichia / Ingeniería Metabólica / Sistemas CRISPR-Cas Idioma: En Revista: Metab Eng Asunto de la revista: ENGENHARIA BIOMEDICA / METABOLISMO Año: 2018 Tipo del documento: Article País de afiliación: Austria