Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization.
Connect Tissue Res
; 59(sup1): 42-45, 2018 12.
Article
en En
| MEDLINE
| ID: mdl-29745809
ABSTRACT
Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs). MATERIAL AND METHODS:
hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.RESULTS:
Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.CONCLUSIONS:
We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Calcificación Fisiológica
/
Diferenciación Celular
/
Colágeno
/
Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
En
Revista:
Connect Tissue Res
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia