Bidirectional modulation of insulin action by reactive oxygen species in 3T3L1 adipocytes.
Mol Med Rep
; 18(1): 807-814, 2018 Jul.
Article
en En
| MEDLINE
| ID: mdl-29767231
ABSTRACT
Reactive oxygen species (ROS) serve an important role in glucoselipid metabolic regulation. In the present study, the results demonstrated that there was bidirectional regulation of insulin action in 3T3L1 adipocytes treated with ROS. Transient and acute ROS exposure improved insulininduced metabolic effects in 3T3L1 adipocytes. Hydrogen peroxide (H2O2), as a stable and diffusible ROS, diffused into adipocytes and altered intracellular redox homeostasis, resulting in oxidation and inactivation of phosphatase and tensin homologue deleted on chromosome 10 (PTEN). Inactivation of PTEN enhanced the activation of insulininduced protein kinase B (AKT), leading to increased glucose transporter 4 (GLUT4) redistribution and glucose uptake in 3T3L1 adipocytes. However, chronic ROS treatment induced insulin resistance in 3T3L1 adipocytes. It was also revealed that insulininduced AKT activation, GLUT4 translocation to cell membrane and glucose uptake were significantly inhibited in chronic ROStreated 3T3L1 adipocytes. Taken together, the present study provided further demonstration that transient ROS treatment improved insulin sensitivity; however, chronic ROS exposure induced insulin resistance in 3T3L1 adipocytes.
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Bases de datos:
MEDLINE
Asunto principal:
Resistencia a la Insulina
/
Adipocitos
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Peróxido de Hidrógeno
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Insulina
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2018
Tipo del documento:
Article