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CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci.
Tasan, Ipek; Sustackova, Gabriela; Zhang, Liguo; Kim, Jiah; Sivaguru, Mayandi; HamediRad, Mohammad; Wang, Yuchuan; Genova, Justin; Ma, Jian; Belmont, Andrew S; Zhao, Huimin.
Afiliación
  • Tasan I; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Sustackova G; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Zhang L; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Kim J; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Sivaguru M; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • HamediRad M; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Wang Y; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Genova J; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
  • Ma J; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  • Belmont AS; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
  • Zhao H; Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Nucleic Acids Res ; 46(17): e100, 2018 09 28.
Article en En | MEDLINE | ID: mdl-29912475
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Proteínas Portadoras / Hibridación Fluorescente in Situ / Técnicas de Sustitución del Gen / Sistemas CRISPR-Cas / Imagen Individual de Molécula Límite: Humans Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Proteínas Portadoras / Hibridación Fluorescente in Situ / Técnicas de Sustitución del Gen / Sistemas CRISPR-Cas / Imagen Individual de Molécula Límite: Humans Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos