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Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNAIle.
Wilcox, Brendan; Osterman, Ilya; Serebryakova, Marina; Lukyanov, Dmitry; Komarova, Ekaterina; Gollan, Bridget; Morozova, Natalia; Wolf, Yuri I; Makarova, Kira S; Helaine, Sophie; Sergiev, Petr; Dubiley, Svetlana; Borukhov, Sergei; Severinov, Konstantin.
Afiliación
  • Wilcox B; Centre for Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 143025, Russia.
  • Osterman I; Centre for Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 143025, Russia.
  • Serebryakova M; Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow 119992, Russia.
  • Lukyanov D; Centre for Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 143025, Russia.
  • Komarova E; Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow 119992, Russia.
  • Gollan B; Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow 119992, Russia.
  • Morozova N; Centre for Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 143025, Russia.
  • Wolf YI; Lomonosov Moscow State University, A.N. Belozersky Institute of Physico-Chemical Biology, Moscow 119992, Russia.
  • Makarova KS; MRC Centre for Molecular Bacteriology and Infection, Flowers Building, Armstrong Road, Imperial College London, London SW7 2AZ, UK.
  • Helaine S; Centre for Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 143025, Russia.
  • Sergiev P; Peter the Great St. Petersburg State Polytechnic University, St. Petersburg, Russia.
  • Dubiley S; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
  • Borukhov S; National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20894, USA.
  • Severinov K; MRC Centre for Molecular Bacteriology and Infection, Flowers Building, Armstrong Road, Imperial College London, London SW7 2AZ, UK.
Nucleic Acids Res ; 46(15): 7873-7885, 2018 09 06.
Article en En | MEDLINE | ID: mdl-29931259
ABSTRACT
Prokaryotic toxin-antitoxin (TA) modules are highly abundant and are involved in stress response and drug tolerance. The most common type II TA modules consist of two interacting proteins. The type II toxins are diverse enzymes targeting various essential intracellular targets. The antitoxin binds to cognate toxin and inhibits its function. Recently, TA modules whose toxins are GNAT-family acetyltransferases were described. For two such systems, the target of acetylation was shown to be aminoacyl-tRNA the TacT toxin targets aminoacylated elongator tRNAs, while AtaT targets the amino acid moiety of initiating tRNAMet. We show that the itaRT gene pair from Escherichia coli encodes a TA module with acetyltransferase toxin ItaT that specifically and exclusively acetylates Ile-tRNAIle thereby blocking translation and inhibiting cell growth. ItaT forms a tight complex with the ItaR antitoxin, which represses the transcription of itaRT operon. A comprehensive bioinformatics survey of GNAT acetyltransferases reveals that enzymes encoded by validated or putative TA modules are common and form a distinct branch of the GNAT family tree. We speculate that further functional analysis of such TA modules will result in identification of enzymes capable of specifically targeting many, perhaps all, aminoacyl tRNAs.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Acetiltransferasas / Toxinas Bacterianas / ARN de Transferencia de Isoleucina / Antitoxinas / Proteínas de Escherichia coli Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article País de afiliación: Rusia

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Acetiltransferasas / Toxinas Bacterianas / ARN de Transferencia de Isoleucina / Antitoxinas / Proteínas de Escherichia coli Idioma: En Revista: Nucleic Acids Res Año: 2018 Tipo del documento: Article País de afiliación: Rusia