A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings.
Biotechniques
; 64(6): 255-261, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29939090
ABSTRACT
One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsis seedlings, cotyledons and leaves. Liquid in the imaging chamber can be rapidly exchanged to introduce chemical treatments via microfluidic passive pumping. When used in conjunction with fluorescent biosensors, this system can facilitate live-cell imaging studies of signal transduction pathways triggered by different treatments. As a demonstration, we show how the HybriWell can be used to monitor flg22-induced calcium transients using the R-GECO1 calcium indicator in detached Arabidopsis leaves.
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Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas Citológicas
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Arabidopsis
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Técnicas Analíticas Microfluídicas
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Dispositivos Laboratorio en un Chip
Idioma:
En
Revista:
Biotechniques
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos