MiR-381-3p inhibits proliferation, migration and invasion by targeting LRP6 in papillary thyroid carcinoma.
Eur Rev Med Pharmacol Sci
; 22(12): 3804-3811, 2018 06.
Article
en En
| MEDLINE
| ID: mdl-29949156
ABSTRACT
OBJECTIVE:
MiR-381-3p plays an essential role in the progression of a variety of cancers, but its expression and role in papillary thyroid carcinoma (PTC) progression have not been investigated. The aim of this study was to investigate the expression of miR-381-3p and its function in PTC. PATIENTS ANDMETHODS:
The expression levels of miR-381-3p and low-density lipoprotein receptorrelated protein 6 (LRP6) mRNA in PTC tissues and cell lines were measured using RT-PCR. Cell proliferation, migration and invasion were assessed by cell viability assay and transwell assay. Luciferase assays and Western blotting were performed to demonstrate miR-381-3p target gene.RESULTS:
We found that miR-381-3p was significantly down-regulated in PTC tissues and cell lines. In vitro assay indicated that up-regulation of miR-381-3p significantly suppressed PTC cell proliferation, migration and invasion. Moreover, luciferase reporter gene assay demonstrated that miR-381-3p could target LRP6 by binding to the 3' UTR. Western blot and Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) showed that miR-381-3p overexpression suppressed the expression of LRP6 at both mRNA and proteins levels. In addition, functional experiment confirmed that LRP6 was involved in the suppressive effect of miR-381-3p-mediated PTC on cell proliferation, migration and invasion.CONCLUSIONS:
Our findings suggested, for the first time, that miR-381-3p was lowly expressed in PTC tissues, and its up-regulation inhibited tumorigenesis of PTC by targeting LRP6.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Tiroides
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Carcinoma Papilar
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MicroARNs
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Proliferación Celular
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Proteína-6 Relacionada a Receptor de Lipoproteína de Baja Densidad
Límite:
Humans
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Asunto de la revista:
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
China