CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.
J Immunol
; 201(4): 1174-1185, 2018 08 15.
Article
en En
| MEDLINE
| ID: mdl-29987160
ABSTRACT
Ca2+ release-activated Ca2+ channel regulator 2A (CRACR2A) is expressed abundantly in T cells and acts as a signal transmitter between TCR stimulation and activation of the Ca2+/NFAT and JNK/AP1 pathways. CRACR2A has been linked to human diseases in numerous genome-wide association studies and was shown to be one of the most sensitive targets of the widely used statin drugs. However, the physiological role of CRACR2A in T cell functions remains unknown. In this study, using transgenic mice for tissue-specific deletion, we show that CRACR2A promotes Th1 responses and effector function of Th17 cells. CRACR2A was abundantly expressed in Th1 and Th17 cells. In vitro, deficiency of CRACR2A decreased Th1 differentiation under nonpolarizing conditions, whereas the presence of polarizing cytokines compensated this defect. Transcript analysis showed that weakened TCR signaling by deficiency of CRACR2A failed to promote Th1 transcriptional program. In vivo, conditional deletion of CRACR2A in T cells alleviated Th1 responses to acute lymphocytic choriomeningitis virus infection and imparted resistance to experimental autoimmune encephalomyelitis. Analysis of CNS from experimental autoimmune encephalomyelitis-induced mice showed impaired effector functions of both Th1 and Th17 cell types, which correlated with decreased pathogenicity. Collectively, our findings demonstrate the requirement of CRACR2A-mediated TCR signaling in Th1 responses as well as pathogenic conversion of Th17 cells, which occurs at the site of inflammation.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al Calcio
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Infecciones por Arenaviridae
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Células TH1
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Encefalomielitis Autoinmune Experimental
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Células Th17
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Virus de la Coriomeningitis Linfocítica
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Immunol
Año:
2018
Tipo del documento:
Article