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Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells.
Lynch, Adam T; Mazzotta, Silvia; Hoppler, Stefan.
Afiliación
  • Lynch AT; Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen, Scotland, UK.
  • Mazzotta S; Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen, Scotland, UK.
  • Hoppler S; Institute of Medical Sciences, Foresterhill Health Campus, University of Aberdeen, Aberdeen, Scotland, UK. s.p.hoppler@abdn.ac.uk.
Methods Mol Biol ; 1816: 55-66, 2018.
Article en En | MEDLINE | ID: mdl-29987810
In vitro generated mammalian cardiomyocytes provide experimental models for studying normal mammalian cardiomyocyte development, for disease modeling and for drug development. They also promise to inform future therapeutic strategies for repair of injured or diseased myocardium. Here we provide reliable protocols for differentiation of mouse embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimizing such protocols for specific cell lines.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Miocitos Cardíacos / Células Madre Embrionarias de Ratones Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Miocitos Cardíacos / Células Madre Embrionarias de Ratones Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2018 Tipo del documento: Article