The expression signature of very long non-coding RNA in myalgic encephalomyelitis/chronic fatigue syndrome.
J Transl Med
; 16(1): 231, 2018 08 17.
Article
en En
| MEDLINE
| ID: mdl-30119681
ABSTRACT
BACKGROUND:
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic debilitating disease with huge social-economic impact. It has been suggested that immune dysregulation, nitrooxidative stress, and metabolic impairment might contribute to disease pathogenesis. However, the etiology of ME/CFS remains largely unclear, and diagnostic/prognostic disease markers are lacking. Several long noncoding RNAs (lncRNA, > 200 bp) have been reported to play roles in immunological diseases or in stress responses.METHODS:
In our study, we examined the expression signature of 10 very long lncRNAs (> 5 kb, CR933609, His-RNA, AK124742, GNAS1-AS, EmX2OS, MIAT, TUG1, NEAT1, MALAT1, NTT) in the peripheral blood mononuclear cells of 44 ME/CFS patients.RESULTS:
LncRNAs NTT, MIAT and EmX2OS levels were found to be significantly elevated in ME/CFS patients as compared with healthy controls. Furthermore, NTT and EmX2OS levels increased with disease severity. Stimulation of human monocytic cell line THP-1 and glioma cell line KALS1 with H2O2 (oxidative stress) and poly (IC) (double strand RNA, representing viral activation) increased the expression levels of NTT and MIAT.CONCLUSIONS:
Our study revealed a ME/CFS-associated very long lncRNA expression signature, which might reflect the regulatory response in ME/CFS patients to oxidative stress, chronic viral infection and hypoxemia. Further investigations need to be done to uncover the functions and potential diagnostic value of these lncRNAs in ME/CFS.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Leucocitos Mononucleares
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Síndrome de Fatiga Crónica
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Perfilación de la Expresión Génica
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ARN Largo no Codificante
Tipo de estudio:
Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Transl Med
Año:
2018
Tipo del documento:
Article
País de afiliación:
Taiwán