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Development of Human Mast Cells from Hematopoietic Stem Cells within a 3D Collagen Matrix: Effect of Stem Cell Media on Mast Cell Generation.
Derakhshan, Tahereh; Bhowmick, Rudra; Ritchey, Jerry W; Gappa-Fahlenkamp, Heather.
Afiliación
  • Derakhshan T; School of Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, USA.
  • Bhowmick R; School of Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, USA.
  • Ritchey JW; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA.
  • Gappa-Fahlenkamp H; School of Chemical Engineering, Oklahoma State University, 420 Engineering North, Stillwater, OK 74078, USA.
Stem Cells Int ; 2018: 2136193, 2018.
Article en En | MEDLINE | ID: mdl-30123284
ABSTRACT
Mast cells (MCs) arise from hematopoietic stem cells (HSCs) that mature within vascularized tissues. Fibroblasts and endothelial cells (ECs) play a role in the maturation of HSCs in the tissues. Due to difficulties in isolating MCs from tissues, large numbers of committed MC precursors can be generated in 2D culture systems with the use of differentiation factors. Since MCs are tissue-resident cells, the development of a 3D tissue-engineered model with ancillary cells that more closely mimics the 3D in vivo microenvironment has greater relevance for MC studies. The goals of this study were to show that MCs can be derived from HSCs within a 3D matrix and to determine a media to support MCs, fibroblasts, and ECs. The results show that HSCs within a collagen matrix cultured in StemSpan media with serum added at the last week yielded a greater number of c-kit+ cells and a greater amount of histamine granules compared to other media tested. Media supplemented with serum were necessary for EC survival, while fibroblasts survived irrespective of serum with higher cell yields in StemSpan. This work demonstrates the development of functional MCs within a 3D collagen matrix using a stem cell media that supports fibroblast and ECs.

Texto completo: 1 Bases de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Stem Cells Int Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Bases de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Stem Cells Int Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos