Amino-Acid-Catalyzed Direct Aldol Bioconjugation.
Org Lett
; 20(17): 5344-5347, 2018 09 07.
Article
en En
| MEDLINE
| ID: mdl-30125118
ABSTRACT
A site-specific bioconjugation was developed based on direct aldol coupling using amino-acid-derived organocatalysts. The functionalization exhibits fast kinetics and occurs under mild, biocompatible conditions (viz., aqueous media, moderate temperature, and neutral pH). The resulting bioconjugates were found to be stable toward abundant aldolase enzymes, as well as acidic and basic pH. The methodology was demonstrated through conjugation of a variety of small molecules, dyes, and peptides to proteins, including a single-domain antibody, which was then used for cellular imaging.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Aldehídos
/
Aminoácidos
Idioma:
En
Revista:
Org Lett
Asunto de la revista:
BIOQUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos