Bacteria-Based Materials for Stem Cell Engineering.
Adv Mater
; 30(43): e1804310, 2018 Oct.
Article
en En
| MEDLINE
| ID: mdl-30209838
ABSTRACT
Materials can be engineered to deliver specific biological cues that control stem cell growth and differentiation. However, current materials are still limited for stem cell engineering as stem cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal stem cells (hMSCs), in a highly dynamic-temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports stem cell growth, whereas inducible-temporal regulation of secreted bone morphogenetic protein-2 drives osteogenesis in an on-demand manner. This approach enables stem cell technologies using material systems that host symbiotic interactions between eukaryotic and prokaryotic cells.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Lactococcus lactis
/
Materiales Biomiméticos
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Células Madre Mesenquimatosas
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Ingeniería Celular
Límite:
Humans
Idioma:
En
Revista:
Adv Mater
Asunto de la revista:
BIOFISICA
/
QUIMICA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Reino Unido