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TGF-ß1-Induced Airway Smooth Muscle Cell Proliferation Involves TRPM7-Dependent Calcium Influx via TGFßR/SMAD3.

Chen, Ming; Zhang, Wei; Shi, Jianting; Jiang, Shanping.
Mol Immunol; 103: 173-181, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-30290314

BACKGROUND:

TRPM7 in mast cells plays a key role in asthma. However, the effect of TRPM7 on TGF-ß1-induced airway smooth muscle cell (ASMC) proliferation remains unclear. Therefore, we designed this study to explore whether TRPM7 is involved in TGF-ß1-induced ASMC proliferation.

METHODS:

An asthmatic mouse model was established, and the expressions of TGF-ß1 and TRPM7 in mice lungs were detected using enzyme-linked immunosorbent assay (ELISA) and western blotting. In addition, murine ASMCs were cultured and stimulated with TGF-ß1. Possible TGF-ß1 and TRPM7 interactions were examined using RT-PCR and western blotting analyses with ASMCs. The effect of TRPM7 knockdown on ASMC calcium influx was assessed by the fluorescent indicator indo-1. MTT and flow cytometry were applied to evaluate the effects of TRPM7 knockdown on ASMC proliferation and apoptosis.

RESULTS:

TGF-ß1 elevated the expression of TRPM7 via TGFßR/SMAD3. Knocking down TRPM7 decreased the intracellular Ca2+ concentration and cellular proliferation of ASMCs, although apoptosis remained unaffected.

CONCLUSIONS:

Our initial findings suggest that TRPM7 inhibition may prevent asthma-induced airway remodeling.