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Backbone resonance assignment for the N-terminal region of bacterial tRNA-(N1G37) methyltransferase.
Li, Yan; Zhong, Wenhe; Koay, Ann Zhufang; Ng, Hui Qi; Koh-Stenta, Xiaoying; Nah, Qianhui; Lim, Siau Hoi; Larsson, Andreas; Lescar, Julien; Hill, Jeffrey; Dedon, Peter C; Kang, CongBao.
Afiliación
  • Li Y; Experimental Therapeutics Centre, 31 Biopolis Way, #03-01 Nanos, Singapore, 138669, Singapore.
  • Zhong W; Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, 430030, People's Republic of China.
  • Koay AZ; Infectious Disease and Antimicrobial Resistance Interdisciplinary Research Groups, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore, 138602, Singapore.
  • Ng HQ; Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore, 138602, Singapore.
  • Koh-Stenta X; Experimental Therapeutics Centre, 31 Biopolis Way, #03-01 Nanos, Singapore, 138669, Singapore.
  • Nah Q; Experimental Therapeutics Centre, 31 Biopolis Way, #03-01 Nanos, Singapore, 138669, Singapore.
  • Lim SH; Experimental Therapeutics Centre, 31 Biopolis Way, #03-01 Nanos, Singapore, 138669, Singapore.
  • Larsson A; Infectious Disease and Antimicrobial Resistance Interdisciplinary Research Groups, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore, 138602, Singapore.
  • Lescar J; Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore, 138602, Singapore.
  • Hill J; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
  • Dedon PC; Experimental Biotherapeutics Centre, 30 Biopolis Street, #08-01 Matrix, Singapore, 138671, Singapore.
  • Kang C; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
Biomol NMR Assign ; 13(1): 49-53, 2019 04.
Article en En | MEDLINE | ID: mdl-30298375
ABSTRACT
Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) is an important antibacterial target due to its essential role in translation. TrmD has two domains connected with a flexible linker. The N-terminal domain (NTD) of TrmD contains the S-adenosyl-L-methionine (SAM) cofactor binding site and the C-terminal domain is critical for tRNA binding. Here we report the backbone NMR resonance assignments for NTD of Pseudomonas aeruginosa TrmD. Its secondary structure was determined based on the assigned resonances. Relaxation analysis revealed that NTD existed as dimers in solution. NTD also exhibited thermal stability in solution. Its interactions with SAM and other compounds suggest it can be used for evaluating SAM competitive inhibitors by NMR.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / ARNt Metiltransferasas / Resonancia Magnética Nuclear Biomolecular Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2019 Tipo del documento: Article País de afiliación: Singapur
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / ARNt Metiltransferasas / Resonancia Magnética Nuclear Biomolecular Idioma: En Revista: Biomol NMR Assign Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA NUCLEAR Año: 2019 Tipo del documento: Article País de afiliación: Singapur